1T1E

High Resolution Crystal Structure of the Intact Pro-Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-01
Detector	MARRESEARCH
Wavelength(s)	1.050
Spacegroup name	H 3 2
Unit cell lengths	158.640, 158.640, 106.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.180
Rwork	0.213
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Native Kumamolisin Catalytic Domain
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.180
Number of reflections	164763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	tri-sodium citrate dihydrate, HEPES-Na, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K