1T16

Crystal structure of the bacterial fatty acid transporter FadL from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	100.380, 68.664, 106.024
Unit cell angles	90.00, 96.37, 90.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.262
Rwork	0.257
R-free	0.30100
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.120
High resolution limit [Å]	2.490
Number of reflections	46492
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	PEG 4000, Copper(II)chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	PEG 4000, Copper(II)chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K