1T10
Phosphoglucose isomerase from Leishmania mexicana in complex with substrate D-fructose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Detector technology | CCD |
| Collection date | 2003-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.129, 85.129, 350.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.530 - 2.350 |
| R-factor | 0.22228 |
| Rwork | 0.220 |
| R-free | 0.25983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.09) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.536 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.339 | |
| Number of reflections | 32626 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 10% PEG 6000, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
