1T0U
Crystal structure of E.coli uridine phosphorylase at 2.2 A resolution (Type-A Native)
Replaces: 1LGGExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 151.336, 151.336, 48.366 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.110 - 2.200 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.20300 |
| Structure solution method | SIR(URANYL DERIVATIVE) |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.831 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.110 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | 0.281 |
| Number of reflections | 20914 | |
| <I/σ(I)> | 15.08 | 4.42 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.77 | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Tris-HCl, PEG, Sodium Acetate, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
