1T0K
Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex
Replaces: 1CK5Replaces: 1CK8Replaces: 1CN8Replaces: 1CN9Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 136.006, 136.006, 123.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 3.240 |
| R-factor | 0.269 |
| Rwork | 0.261 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.555 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 3.410 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Number of reflections | 18636 | |
| Completeness [%] | 98.2 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | Lithium Sulfate, Sodium Cacodylate, Magnesium Chloride, Jeffamine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Lithium Sulfate | ||
| 2 | 1 | 1 | Sodium Cacodylate | ||
| 3 | 1 | 1 | Magnesium Chloride | ||
| 4 | 1 | 1 | Jeffamine |
