1T0H
Crystal structure of the Rattus norvegicus voltage gated calcium channel beta subunit isoform 2a
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0199, 0.9796 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.176, 45.260, 58.701 |
| Unit cell angles | 107.34, 95.75, 97.00 |
Refinement procedure
| Resolution | 55.050 - 1.970 |
| R-factor | 0.18693 |
| Rwork | 0.185 |
| R-free | 0.21321 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 24274 | |
| <I/σ(I)> | 27.51 | 2.45 |
| Completeness [%] | 100.0 | 96.5 |
| Redundancy | 5.19 | 2.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 281 | Tris-Cl, NaCl, PEG 4000, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
