1T07
Crystal Structure of Conserved Protein of Unknown Function PA5148 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-24 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 55.574, 55.574, 62.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.090 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.192 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.102 | 0.572 |
| Number of reflections | 10400 | |
| <I/σ(I)> | 7.5 | 2.6 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 17.7 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | PEG 3350, Citric acid, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
