1T01

Vinculin complexed with the VBS1 helix from talin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 2
Unit cell lengths	51.645, 71.421, 96.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.060
Rwork	0.230
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	24132
<I/σ(I)>		2.509
Completeness [%]	98.4	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	30%PEG 5000, 0.1M MES, 0.2M Ammonium Sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.50