1SZT

ATOMIC STRUCTURE OF A THERMOSTABLE SUBDOMAIN OF HIV-1 GP41

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-02-26
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	53.070, 53.070, 60.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.208
Rwork	0.208
R-free	0.26900
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	0.999
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.057	0.211
Number of reflections	2481
<I/σ(I)>	27.61	8.98
Completeness [%]	99.6	99.6
Redundancy	8.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	20 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	20 (%)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	glycerol	10 (%)
5	1	reservoir		0.1 (M)