1SZP
A Crystal Structure of the Rad51 Filament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 |
| Unit cell lengths | 135.262, 135.262, 128.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.250 |
| R-factor | 0.27286 |
| Rwork | 0.268 |
| R-free | 0.31978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n0w |
| RMSD bond length | 0.043 |
| RMSD bond angle | 2.846 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.250 |
| Number of reflections | 41066 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | NaCitrate, PEG 5000-MME, Xylitol, Ammmonium Sulfate, Sodium Chloride, Magnesium Chloride, Dithiotritol, Sodium Azide, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
