1SZF
A198G:L230A mutant flavocytochrome b2 with pyruvate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 163.413, 163.413, 112.425 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.000 - 2.700 |
| R-factor | 0.22155 |
| Rwork | 0.220 |
| R-free | 0.25331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb id 1fcb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.438 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.071 | 0.202 |
| Number of reflections | 47555 | |
| <I/σ(I)> | 14.8 | 4.8 |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 291 | 0.1 M citrate, 200 mM NaBr, 12-15 % PEG3350, 18-20% glycerol, 50 mM pyruvate, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
