1SZB
Crystal structure of the human MBL-associated protein 19 (MAp19)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.946 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.754, 67.754, 187.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.500 |
| R-factor | 0.263 |
| Rwork | 0.261 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nt0 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15712 | |
| <I/σ(I)> | 23.7 | 5.1 |
| Completeness [%] | 94.8 | 86.1 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
