1SZ7
Crystal structure of Human Bet3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978573 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.901, 72.901, 56.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| Rwork | 0.207 |
| R-free | 0.24516 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 24929 | |
| <I/σ(I)> | 20.3 | 2 |
| Completeness [%] | 97.0 | 89.2 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG400, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
