1SZ6
MISTLETOE LECTIN I FROM VISCUM ALBUM. CRYSTAL STRUCTURE AT 2.05 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84530 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 106.880, 106.880, 310.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.000 - 2.050 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mll |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.044 | 0.299 |
| Number of reflections | 65558 | |
| <I/σ(I)> | 19.2 | 3.82 |
| Completeness [%] | 98.2 | 96.1 |
| Redundancy | 5.46 | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 293 | 40% SATURATED AMMONIUM SULPHATE, 30% GLYCEROL IN 0.1M, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
