1SYY
Crystal structure of the R2 subunit of ribonucleotide reductase from Chlamydia trachomatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-09-30 |
Detector | MACSCIENCE |
Wavelength(s) | 1.0980 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.738, 62.738, 171.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.155 |
Rwork | 0.153 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 2.150 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 38503 | |
Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 20000, MES, Pb(CH3COO)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |