1SYT
Crystal structure of signalling protein from goat SPG-40 in the presense of N,N',N''-triacetyl-chitotriose at 2.6A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.111, 66.036, 107.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.600 |
| R-factor | 0.18143 |
| Rwork | 0.180 |
| R-free | 0.21703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QZO |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.725 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 13563 | |
| <I/σ(I)> | 11.5 | 3.1 |
| Completeness [%] | 96.1 | 98.4 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25mM Tris-HCl, 50mM NACl, 19% ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
