1SY7
Crystal structure of the catalase-1 from Neurospora crassa, native structure at 1.75A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.782 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.007, 182.242, 90.364 |
| Unit cell angles | 90.00, 133.41, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.750 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gge |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.090 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 153020 | |
| <I/σ(I)> | 8.6 | 2.3 |
| Completeness [%] | 89.0 | 89.8 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | Ammonium sulfate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
