1SY2
1.0 A Crystal Structure of D129A/L130A Mutant of Nitrophorin 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.149, 42.804, 52.881 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 21.400 - 1.000 |
| R-factor | 0.1563 |
| Rwork | 0.156 |
| R-free | 0.16960 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.660 |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.400 | 1.030 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.049 | 0.157 |
| Number of reflections | 85962 | |
| <I/σ(I)> | 27.3 | 7.7 |
| Completeness [%] | 98.8 | 99.4 |
| Redundancy | 4.62 | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium Phosphate 2.8 M, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
