1SY0
1.15 A Crystal Structure of T121V Mutant of Nitrophorin 4 from Rhodnius Prolixus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.300, 42.763, 52.890 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
| Resolution | 21.370 - 1.150 |
| R-factor | 0.1428 |
| Rwork | 0.141 |
| R-free | 0.17179 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2u |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDISPLAYF |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.370 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.050 | 0.134 |
| Number of reflections | 54132 | |
| <I/σ(I)> | 20.8 | 6 |
| Completeness [%] | 97.1 | 98.6 |
| Redundancy | 3.19 | 2.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium Phosphate 3.2 M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
