1SXX
1.0 A Crystal Structure of D129A/L130A Mutant of Nitrophorin 4 Complexed with Nitric Oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.751 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.219, 42.751, 53.055 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 30.570 - 1.010 |
| R-factor | 0.14123 |
| Rwork | 0.141 |
| R-free | 0.15498 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1koi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.703 |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 1.040 |
| High resolution limit [Å] | 1.010 | 1.010 |
| Rmerge | 0.049 | 0.239 |
| Number of reflections | 80684 | |
| <I/σ(I)> | 18.5 | 5.2 |
| Completeness [%] | 97.1 | 89.2 |
| Redundancy | 4.1 | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Ammonium Phosphate 3.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
