1SXH
apo structure of B. megaterium transcription regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.680, 72.190, 105.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.910 - 2.750 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27300 |
| Structure solution method | MIR |
| Starting model (for MR) | protein-DNA bound form C-subdomains only |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.910 | 2.920 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 15039 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350, 0.2 M NaCl, HEPES, 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
