1SXG
Structural studies on the apo transcription factor form B. megaterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 375.390, 60.140, 75.650 |
| Unit cell angles | 90.00, 95.59, 90.00 |
Refinement procedure
| Resolution | 75.290 - 2.750 |
| R-factor | 0.2141 |
| Rwork | 0.214 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P212121 apo structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.290 | 2.920 |
| High resolution limit [Å] | 2.710 | 2.750 |
| Number of reflections | 44614 | |
| Completeness [%] | 98.7 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG 3350, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
