1SWX

Crystal structure of a human glycolipid transfer protein in apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-06-25
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	55.439, 35.259, 57.507
Unit cell angles	90.00, 115.82, 90.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.17536
Rwork	0.172
R-free	0.22567
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.694
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.063	0.340
Number of reflections	23776
Completeness [%]	97.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	PEG 3350, potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	PEG 3350, potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K