1SWT

CORE-STREPTAVIDIN MUTANT D128A IN COMPLEX WITH BIOTIN AT PH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-11-20
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	55.700, 84.800, 50.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.156 *
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.006
RMSD bond angle	0.024
Data reduction software	SADIE
Data scaling software	SADABS
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.054
Total number of observations	39212 *
Number of reflections	14716
<I/σ(I)>	7	1
Completeness [%]	88.0	43
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5		INCUBATED IN 2.5 M BIOTIN/HOH, COCRYSTALLIZED IN 52% MPD (PH 4.5)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	MPD	50 (%)