1SWR

CORE-STREPTAVIDIN MUTANT W120A IN COMPLEX WITH BIOTIN AT PH 7.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-12-18
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	52.800, 99.900, 50.900
Unit cell angles	90.00, 112.70, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.184
R-free	0.22600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swe
RMSD bond length	0.006
RMSD bond angle	0.025
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.060 *	0.300
Total number of observations	96477 *
Number of reflections	30564
<I/σ(I)>	12.8	2.8
Completeness [%]	87.0	74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	biotin		2.5 molar excess
3	1	reservoir	PEG4000	12-20 (%)
4	1	reservoir	HEPES	0.1 (M)