1SWQ

CORE-STREPTAVIDIN MUTANT W120A AT PH 7.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-05-31
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	58.200, 87.300, 47.000
Unit cell angles	90.00, 98.80, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.169 *
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.006
RMSD bond angle	0.025
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.020	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.050 *	0.163
Total number of observations	52682 *
Number of reflections	29377
<I/σ(I)>	11.9	4.3
Completeness [%]	76.0 *	71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	PEG4000	12-20 (%)
3	1	reservoir	HEPES	0.1 (M)