1SWO

CORE-STREPTAVIDIN MUTANT W120F AT PH 7.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1994-10-17
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	58.500, 87.200, 47.100
Unit cell angles	90.00, 98.90, 90.00

Refinement procedure

Resolution	10.000 - 1.950
R-factor	0.166 *
R-free	0.24200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.006
RMSD bond angle	0.023
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.040 *	0.114
Total number of observations	53049 *
Number of reflections	26060
<I/σ(I)>	18.7	4.99
Completeness [%]	78.0 *	56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	PEG4000	12-20 (%)
3	1	reservoir	HEPES	0.1 (M)