1SWN

CORE-STREPTAVIDIN MUTANT W108F IN COMPLEX WITH BIOTIN AT PH 7.0

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-11-21
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	59.600, 88.700, 48.000
Unit cell angles	90.00, 98.80, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.16 *
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.005
RMSD bond angle	0.020
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.056	0.330
Total number of observations	84710 *
Number of reflections	19696
<I/σ(I)>	13.8	3.1
Completeness [%]	87.0	35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	biotin		2.5 molar excess
3	1	reservoir	PEG1000	22-24 (%)
4	1	reservoir	Tris-HCl	0.1 (M)