1SWJ

CORE-STREPTAVIDIN MUTANT W79F AT PH 4.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1994-04-19
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	47.300, 76.400, 70.200
Unit cell angles	90.00, 100.90, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.151 *
R-free	0.25500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.005
RMSD bond angle	0.022
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.023	0.126
Total number of observations	26263 *
Number of reflections	20966
<I/σ(I)>	15.6	3.8
Completeness [%]	79.0	65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	MPD	(%)