1SWG

CIRCULAR PERMUTED STREPTAVIDIN E51/A46 IN COMPLEX WITH BIOTIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	71.900, 78.600, 90.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.19
R-free	0.23100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SWA (EXCLUDING RESIDUES 46 - 51 IN ALL SUBUNITS)
RMSD bond length	0.007
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.030 *	0.170 *
Total number of observations	417564 *
Number of reflections	51393
<I/σ(I)>	19.9	2
Completeness [%]	98.2 *	96.2 *
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		PROTEIN (12MG/ML, 10MM BIOTIN) WAS CRYSTALLIZED FROM 52% MPD (PH 4.5)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	biotin	10 (mM)
3	1	reservoir	MPD	52 (%)