1SWD
APO-CORE-STREPTAVIDIN IN COMPLEX WITH BIOTIN (TWO UNOCCUPIED BINDING SITES) AT PH 4.5
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-04-23 |
| Detector | NICOLET |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.794, 85.191, 46.599 |
| Unit cell angles | 90.00, 100.13, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.202 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.026 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | SHELXL-96 |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.417 |
| Number of reflections | 25758 | |
| <I/σ(I)> | 14 | 1.1 |
| Completeness [%] | 76.0 | 20 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | PROTEIN-BIOTIN COMPLEX WAS CO-CRYSTALLIZED FROM 50% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5) WITH 1.2M EXCESS OF BIOTIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | drop | MPD | 60 (%) | |
| 3 | 1 | drop | biotin | 1.2 (M) | excess |
| 4 | 1 | reservoir | MPD | 50 (%) |
