1SWB
APO-CORE-STREPTAVIDIN AT PH 7.5
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-11-28 |
| Detector | NICOLET |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.900, 88.200, 47.400 |
| Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| R-factor | 0.17 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.024 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.840 | 1.900 |
| Rmerge | 0.052 | 0.178 |
| Number of reflections | 30418 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 78.0 | 48 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | PROTEIN WAS CRYSTALLIZED FROM 48% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5), 5.5H SOAKING IN 0.1M HEPES BUFFER PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 48 (%) |
