1SW7
Triosephosphate isomerase from Gallus gallus, loop 6 mutant K174N, T175S, A176S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.218, 87.218, 162.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.220 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.23589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sw3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.309 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 31858 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 2.6M ammonium sulfate, 0.1M Na-citrate, 0.2M NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
