1SW4
Crystal structure of ProX from Archeoglobus fulgidus in complex with trimethyl ammonium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.730, 75.740, 66.980 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.2176 |
| Rwork | 0.216 |
| R-free | 0.24544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sw1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.309 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 49535 | |
| <I/σ(I)> | 9.7 | 4.1 |
| Completeness [%] | 98.6 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | zinc acetate, sodium cacodylate, PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
