1SW3
Triosephosphate isomerase from Gallus gallus, loop 6 mutant T175V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.443, 73.850, 136.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.030 |
| R-factor | 0.14454 |
| Rwork | 0.143 |
| R-free | 0.17526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sw0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.369 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 37515 |
| Completeness [%] | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 25% PEG 6000, 0.2M Li2SO4, 0.1M ADA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
