1SW1
Crystal structure of ProX from Archeoglobus fulgidus in complex with proline betaine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0440, 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.400, 78.400, 67.800 |
| Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18245 |
| Rwork | 0.180 |
| R-free | 0.21890 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.434 |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 52348 | |
| <I/σ(I)> | 13.8 | 5.4 |
| Completeness [%] | 98.4 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | zinc acetate, sodium cacodylate, PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
