1SW0
Triosephosphate isomerase from Gallus gallus, loop 6 hinge mutant K174L, T175W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.744, 73.447, 135.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.180 - 1.710 |
| R-factor | 0.19812 |
| Rwork | 0.197 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tph |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.471 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.180 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 47599 | |
| Completeness [%] | 62.6 | 23.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.4M Na-citrate, 0.1M TEA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
