1SVY
SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.352, 49.218, 62.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.1838 |
| Rwork | 0.184 |
| R-free | 0.24620 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES (V. 95) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.035 | 0.116 |
| Number of reflections | 10235 | |
| <I/σ(I)> | 30.9 | 9.9 |
| Completeness [%] | 93.1 | 77.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 1.5 (M) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | drop | protein | 20 (mg/ml) |
