1SVV

Initial Stuctural Analysis of Leishmania major Threonine Aldolase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794, 0.9494, 0.9797
Spacegroup name	P 43 21 2
Unit cell lengths	119.094, 119.094, 129.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.170 - 2.100
R-factor	0.19927
Rwork	0.198
R-free	0.22968
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.266
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.580
High resolution limit [Å]	2.100
Number of reflections	54773
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	PEG8000, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.50