1SVF
PARAMYXOVIRUS SV5 FUSION PROTEIN CORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 |
| Unit cell lengths | 41.600, 41.600, 95.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.400 |
| R-factor | 0.181 * |
| Rwork | 0.181 |
| R-free | 0.20200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.063 | 0.274 |
| Total number of observations | 81223 * | |
| Number of reflections | 35812 | 3498 * |
| <I/σ(I)> | 15.1 | 2 |
| Completeness [%] | 98.1 | 95.8 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 7-9% POLYETHYLENE GLYCOL 0.5-0.6M LITHIUM SULFATE, pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 7-9 (%) | |
| 4 | 1 | reservoir | lithium sulfate | 0.5-0.6 (M) |
