1SV6
Crystal structure of 2-hydroxypentadienoic acid hydratase from Escherichia Coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-02-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.222, 121.507, 111.796 |
Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.243 |
Rwork | 0.239 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.320 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 31828 |
Completeness [%] | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 4000, isopropanol, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |