1SV3
Structure of the complex formed between Phospholipase A2 and 4-methoxybenzoic acid at 1.3A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 203 |
Detector technology | CCD |
Collection date | 2003-09-29 |
Detector | MARRESEARCH |
Spacegroup name | P 43 |
Unit cell lengths | 52.570, 52.570, 47.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.350 |
R-factor | 0.20986 |
Rwork | 0.208 |
R-free | 0.24267 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fb2 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.781 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.360 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 28771 | |
<I/σ(I)> | 8.8 | 2.1 |
Completeness [%] | 94.1 | 99.4 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 297 | 0.2M Ammonium sulphate, 50% PEG, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |