1SUU

Structure of DNA gyrase A C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-03
Detector	ADSC QUANTUM 210
Wavelength(s)	1.127, 0.9796, 1.0199
Spacegroup name	P 21 21 21
Unit cell lengths	42.523, 82.294, 88.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.750
R-factor	0.19508
Rwork	0.194
R-free	0.22505
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.136
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	29828
<I/σ(I)>	29.6	4.6
Completeness [%]	97.9	91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	sodium phosphate, PEG-3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	sodium phosphate, PEG-3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	sodium phosphate, PEG-3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K