1SUS
Crystal structure of alfalfa feruoyl coenzyme A 3-O-methyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97966, 0.97927, 0.97549 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.854, 136.486, 332.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| Rwork | 0.245 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 37858 | |
| <I/σ(I)> | 19.6 | 0.92 |
| Completeness [%] | 94.2 | 61.8 |
| Redundancy | 2 | 1.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | PEG 8000, TAPS, calcium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
