1SUL
Crystal Structure of the apo-YsxC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.670, 36.520, 83.253 |
| Unit cell angles | 90.00, 122.16, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.17717 |
| Rwork | 0.173 |
| R-free | 0.25084 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.392 |
| Number of reflections | 24474 | |
| <I/σ(I)> | 9.9 | 2.2 |
| Completeness [%] | 93.4 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Sodium acetate, PEG 4000, Tris HCL, MnCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
