1SUA
SUBTILISIN BPN'
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-07 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.530, 60.330, 83.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1suc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.032 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | AMoRE |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.190 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 * | 0.370 * |
| Total number of observations | 39158 * | |
| Number of reflections | 13358 | |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 82.0 | 91 * |
| Redundancy | 3 | 0.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 1.25M LI2SO4 0.1M HEPES/HCL PH7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | HEPES/HCl | 0.055 (M) | |
| 3 | 1 | drop | 0.625 (M) | ||
| 4 | 1 | reservoir | HEPES/HCl | 0.1 (M) | |
| 5 | 1 | reservoir | 1.25 (M) |
