1STZ

Crystal structure of a hypothetical protein at 2.2 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	115.295, 115.295, 185.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.21709
Rwork	0.215
R-free	0.25753
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.234
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200	2.200
Number of reflections	66929
Completeness [%]		91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	50 mM Sodium Acetate, 2.0 M Sodium Formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K