1STR

STREPTAVIDIN DIMERIZED BY DISULFIDE-BONDED PEPTIDE AC-CHPQNT-NH2 DIMER

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	96.740, 106.040, 48.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.800
R-factor	0.189
Rwork	0.189
R-free	0.23300
RMSD bond length	0.018
RMSD bond angle	27.640 *
Data reduction software	SADIE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.740
Rmerge	0.074
Total number of observations	60758 *
Number of reflections	19758
Completeness [%]	74.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	30-32 (%sat)
2	1	reservoir	potassium acetate	0.1 (M)