1STE
STAPHYLOCOCCAL ENTEROTOXIN C2 FROM STAPHYLOCOCCUS AUREUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 43.050, 43.050, 290.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25500 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.980 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.050 |
| Number of reflections | 19302 |
| Completeness [%] | 97.6 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 16 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 25-28 (%) | |
| 2 | 1 | drop | ammonium sulfate | 0.15 (M) | |
| 3 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 4 | 1 | drop | cacodylate | 0.1 (M) | |
| 5 | 1 | reservoir | protain | 2 (mg/ml) |
