1STB

ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	47.160, 47.160, 63.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.196
Rwork	0.196
RMSD bond length	0.015
RMSD bond angle	3.700
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.042 *
Total number of observations	42548 *
Number of reflections	7854 *
Completeness [%]	85.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	potassium phosphate	10.5 (mM)
3	1	drop	MPD	17 (%)
4	1	drop	pdTp	2 (mg/ml)
5	1	drop		10 (mM)
6	1	drop	potassium citrate	20 (mM)
7	1	reservoir	MPD	21-23 (%(w/w))
8	1	reservoir	potassium phosphate	10.5 (mM)